Defined Bioscience’s HiDef-B8 500X Supplement, when added to your basal media of choice (NOT INCLUDED), is a complete, serum-free, defined formulation designed for the feeder-free maintenance and expansion of human induced pluripotent stem cells (iPSC) in the undifferentiated state. The HiDef-B8 500X Supplement is a unique formulation that offers modern conveniences of a flexible feeding schedule (including weekend-free maintenance) and also the ability to choose the matrix and passaging reagent that best suits specific applications. HiDef-B8 contains insulin, ascorbic acid-2-phosphate (AA2P), transferrin, sodium selenite, thermostable FGF2/bFGF (aka FGF2-STAB or FGF2-G3), TGFB3, and NRG1. The addition of further components is not required.

HiDef-B8 has been validated with DMEM/F12 from Corning® (Corning 10-092-CM) and may be used with either Matrigel (Corning) or Cultrex/Geltrex (Trevigen/Gibco) at concentrations as low as 2.5 µg cm^-2 (a 1:800 dilution) or higher. Basal DMEM/F12 media and matrices from other manufactures may also be used once validated in your hands.

Each lot of HiDef-B8 500X Supplement is used to prepare complete iPSC medium and then performance-tested in a culture assay using human iPSCs.