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- Ensure a clean, sterile environment to avoid contamination
- Use the right type of stem cell line (iPSCs) appropriate for your experiments
- Keep the cells in an incubator at optimal temperature and humidity conditions
- Monitor cell growth regularly
- Perform regular cell culture maintenance and passaging
- Use single-use, disposable cultureware to prevent cross-contamination
- Use sterile technique when transferring cells to new plates
- Ensure optimal pH, osmolarity, and cell density
- Provide the right balance of oxygen and carbon dioxide for cell growth
- Use the proper cell culture media and supplements for efficient growth
